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Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 210-239)

Details for Product anti-BECN1 Antibody No. ABIN388546, Supplier: Log in to see
Antigen
  • beclin-1
  • atg6
  • vps30
  • beclin1
  • Beclin-1
  • becn1
  • zgc:73097
  • ATG6
  • VPS30
  • 4921513J16Rik
  • 5430417M23Rik
  • Atg6
Epitope
AA 210-239
33
31
19
14
13
13
13
11
11
9
8
8
8
6
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
347
99
82
13
12
11
10
5
5
3
2
1
1
1
Host
Rabbit
276
74
4
Clonality (Clone)
Polyclonal ()
Conjugate
This Beclin 1 antibody is un-conjugated
20
19
19
19
18
18
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
274
151
120
55
55
46
27
12
12
11
6
2
1
1
Supplier
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Immunogen This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 AA from human Beclin 1.
Clone RB7463
Isotype Ig
Specificity This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217~247 amino acids surrounding amino acid E225 of human BECN1.
Predicted Reactivity Cow (Bovine),Pig (Porcine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Beclin 1 (BECN1 Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
Synonyms: BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin-1, coiled-coil myosin-like BCL2-interacting protein
Molecular Weight 51896 DA
Gene ID 8678
UniProt Q14457
Research Area Apoptosis/Necrosis, Cell Signaling, Cell Structure, Autophagy
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 210-239) (ABIN388546) The anti-BECN1 Pab (ABIN388546) is used in Western blot to detect BECN1 in, from left...
Western Blotting (WB) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 210-239) (ABIN388546) Western blot analysis of anti-hBECN1-E225 Pab (ABIN388546) in 293 cell line lysates t...
Immunohistochemistry (IHC) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 210-239) (ABIN388546) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Autop...
Product cited in: Jia, Jin, Xiao et al.: "Lipoxin A4 methyl ester alleviates vascular cognition impairment by regulating the expression of proteins related to autophagy and ER stress in the rat hippocampus." in: Cellular & molecular biology letters, Vol. 20, Issue 3, pp. 475-87, 2015 (PubMed).

Liu, He, von Rütte et al.: "Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma." in: Science translational medicine, Vol. 5, Issue 202, pp. 202ra123, 2013 (PubMed).

Dhule, Penfornis, Frazier et al.: "Curcumin-loaded ?-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 8, Issue 4, pp. 440-51, 2012 (PubMed).

Wang, Whiteman, Lian et al.: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, 2009 (PubMed).

Background publications Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).