Beclin 1, Autophagy Related (BECN1) antibody

Antigen: Beclin 1, Autophagy Related (BECN1)

Synonyms: ATG6, VPS30, beclin1, MGC6843, 4921513J16Rik, 5430417M23Rik, MGC73097, zgc:73097, BECN1, becn1, MGC80672, MGC126978, atg6, vps30, Beclin-1, DKFZp459C0719

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388546

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Beclin 1

Gene ID: 8678

Swiss-Prot: Q14457

Immunogen: This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217~247 amino acids surrounding amino acid E225 of human BECN1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin-1, coiled-coil myosin-like BCL2-interacting protein

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide surrounding amino acid E225 of human BECN1.

Molecular Weight: 51896 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

The anti-BECN1 Pab (ABIN388546) is used in Western blot to detect BECN1 in, from left to right, A2058, Hela, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified Pab.

Western blot analysis of anti-hBECN1-E225 Pab (ABIN388546) in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-E225(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Autophagy BECN1 (APG6) antibody (E225) (ABIN388546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.