|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Beclin 1, Autophagy Related (BECN1) antibody
|Synonyms||ATG6, VPS30, beclin1, MGC6843, 4921513J16Rik, 5430417M23Rik, MGC73097, zgc:73097, BECN1, becn1, MGC80672, MGC126978, atg6, vps30, Beclin-1, DKFZp459C0719|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA
|6 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||Beclin 1|
|Immunogen||This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217~247 amino acids surrounding amino acid E225 of human BECN1.|
|Description||Other names: BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin-1, coiled-coil myosin-like BCL2-interacting protein|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217~247 amino acids surrounding amino acid E225 of human BECN1.|
|Molecular Weight||51896 DA|
Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Cell Signaling, Cell Structure, Autophagy|
|Restrictions||For Research Use only|
Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).
Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).
Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).
Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).
Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).
Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).
|Hosts||Rabbit (74), Mouse (17), Sheep (2)|
|Reactivities||Human (89), Mouse (Murine) (42), Rat (Rattus) (35), Dog (Canine) (22), Cow (Bovine) (19), Horse (Equine) (18), Pig (Porcine) (18), Monkey (3), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (77), ELISA (36), Immunofluorescence (IF) (34), Immunohistochemistry (IHC) (27), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Flow Cytometry (FACS) (10), Immunocytochemistry (ICC) (9), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (2), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (10), C-Term (7), Internal Region (2), AA 53-72 (1), N-Term,Thr72 (1)|