Autophagy/beclin-1 Regulator 1 (AMBRA1) (N-Term) antibody

Antigen: Autophagy/beclin-1 Regulator 1 (AMBRA1)

Synonyms: Ambra1, AA474864, AV021921, mKIAA1736, A130023A14, RP23-273F8.1, 2310079H06Rik, D030051N19Rik, DCAF3, WDR94, FLJ20294, KIAA1736, MGC33725, AMBRA1, MGC128737

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388572

Additional Information

Alternative name: AMBRA1

Gene ID: 55626

UniProt: Q9C0C7

Immunogen: This AMBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AMBRA1.

Isotype: Ig

Description: Other names: Activating molecule in BECN1-regulated autophagy protein 1, KIAA1736

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AMBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AMBRA1.

Molecular Weight: 142507 DA

Comments:

Background: AMBRA1 regulates autophagy and development of the nervous system. This protein is involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy, Cell Cycle, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of AMBRA1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMBRA1(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with AMBRA1 antibody (N-term) (ABIN388572), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Fimia, Stoykova, Romagnoli et al.: "Ambra1 regulates autophagy and development of the nervous system." in: Nature, Vol. 447, Issue 7148, pp. 1121-5, 2007 (PubMed).