AMBRA1 antibody (C-Term)

Catalog No. ABIN499271

The Rabbit Polyclonal anti-AMBRA1 antibody is suitable to detect AMBRA1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for AMBRA1 antibody (C-Term) (ABIN499271)

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMBRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-AMBRA1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to AMBRA1.

Purification: Affinity chromatography purified via peptide column

Immunogen: Ambra1 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human Ambra1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Ambra1 antibody can be used for the detection of Ambra1 at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for AMBRA1

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Alternative Name: AMBRA1

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death . Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.Synonyms: Activating molecule in BECN1-regulated autophagy protein 1, KIAA1736

Gene ID: 55626

UniProt: Q9C0C7

Pathways: Autophagy