ATG14 Autophagy Related 14 Homolog (S. Cerevisiae) (ATG14) (N-Term) antibody

Antigen: ATG14 Autophagy Related 14 Homolog (S. Cerevisiae) (ATG14)

Synonyms: CRP, CRP1, CSRP, CYRP, D1S181E, DKFZp686M148, Csrp, AA408841, AA959891, AW545626, cb796, zgc:77332, MGC85577, csrp1, MGC89932, CSRP1, crp2, lmo5, smlim, zgc:158737, DKFZp469J0137

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388584

Additional Information

Alternative name: Barkor

Gene ID: 1465

UniProt: P21291

Immunogen: This Barkor antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human Barkor.

Isotype: Ig

Description: Other names: Uncharacterized protein KIAA0831, KIAA0831

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human ATG14.

Molecular Weight: 55309 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of Barkor antibody (N-term) (ABIN388584) in mouse heart tissue lysates (35ug/lane). Barkor (arrow) was detected using the purified Pab.

Barkor Antibody (N-term) (ABIN388584) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Sun, Fan, Chen et al.: "Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 49, pp. 19211-6, 2008 (PubMed).