MAP-2 (C-Term) antibody

Antigen: MAP-2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388759

Additional Information

Alternative name: MAP2

Gene ID: 4133

UniProt: P11137

Immunogen: This MAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 418~447 amino acids from the C-terminal region of human MAP2.

Isotype: Ig

Description: Other names: Microtubule-associated protein 2, MAP-2, MAP2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 418~447 amino acids from the C-terminal region of human MAP2.

Molecular Weight: 199610 DA

Comments:

Background: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The exact function of this gene is still unknown. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Immunohistochemistry~~1:50~100 Western Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hMAP2-Q425 (arrow) using rabbit polyclonal hMAP2-Q425 Antibody (ABIN388759). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the hMAP2-Q425 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with hMAP2-Q425 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistr

Publications

Product: DeTure, Di Noto, Purich: "In vitro assembly of Alzheimer-like filaments. How a small cluster of charged residues in Tau and MAP2 controls filament morphology." in: The Journal of biological chemistry, Vol. 277, Issue 38, pp. 34755-9, 2002 (PubMed).

Al-Bassam, Ozer, Safer et al.: "MAP2 and tau bind longitudinally along the outer ridges of microtubule protofilaments." in: The Journal of cell biology, Vol. 157, Issue 7, pp. 1187-96, 2002 (PubMed).

Liu, Saad, Shen et al.: "Diagnostic value of microtubule-associated protein-2 (MAP-2) for neuroendocrine neoplasms." in: Advances in anatomic pathology, Vol. 10, Issue 2, pp. 101-6, 2003 (PubMed).