Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1) (C-Term) antibody

Antigen: Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1)

Synonyms: ASH1, Mash1, bHLHa46, AI225900, ASCL1, HASH1, MASH1, ash, asha, zasha, zash1a, Zash-1a, MGC109810, id:ibd5085, im:7138587, si:dkey-264k15.2, ascl1, ascl1-A

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388761

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ASCL1 (Achaete-scute homolog 1) (C-term)

Gene ID: 429

Swiss-Prot: NP_004307

Immunogen: This ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176~205 amino acids from the C-terminal region of human Hash1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: KMT2H

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Hash1.

Molecular Weight: 25454 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cancer, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-Hash1 N-term Pab (ABIN388760) is used in Western blot to detect Hash1 in mouse lung tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.