Recombinant ASCL1 antibody (AA 186-236)

Catalog No. ABIN7455113

The Rabbit Monoclonal anti-ASCL1 antibody (Clone BLR164J) (ABIN7455113) specifically detects ASCL1 in WB, IHC, ICC, IP and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant ASCL1 antibody (AA 186-236) (ABIN7455113)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: BLR164J

Product Details anti-ASCL1 Antibody

Binding Specificity: AA 186-236

Purpose: Rabbit anti-ASCL1/MASH1 Recombinant Monoclonal Antibody [BLR164J]

Purification: Purified

Immunogen: between AA 186 and 236 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1/MASH1

Background: ASCL1/MASH1 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. Dimerization with other BHLH proteins is required for efficient DNA binding. ASCL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. [taken from NCBI Entrez Gene (Gene ID: 429)].

Gene ID: 429

UniProt: P50553

Pathways: Dopaminergic Neurogenesis