POU Class 5 Homeobox 1 (POU5F1) (AA 110-141), (Glu125) antibody

Details for Product No. ABIN388789
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, POU5F1, POU5F1L
Epitope
AA 110-141, Glu125
(17), (16), (11), (7), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(203), (84), (45), (29), (29), (28), (8), (5), (3), (3), (2), (2), (1), (1)
Host
Rabbit
(141), (68), (12), (10)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(167), (83), (46), (31), (30), (24), (20), (18), (7), (6), (6), (3), (3), (1), (1)
Pubmed 4 references available
Catalog no. ABIN388789
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 AA from human OCT4 (OCT3).
Clone RB14258
Isotype Ig
Specificity This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~148 amino acids surrounding E125 of human OCT3/4.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name OCT4 (OCT3)
Background Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Synonyms: POU domain class 5 transcription factor 1, POU5F1, Octamer-binding transcription factor 3, OCT-3, OTF3, OCT-4
Molecular Weight 38562 Da
Gene ID 5460
UniProt Q01860
Research Area Stem Cells, Transcription Factors, Signaling
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 110-141), (Glu125) antibody Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35 µg/lane). OCT3/4 (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 110-141), (Glu125) antibody (2) Immunofluorescence analysis of OCT3/4 Antibody (E125) (ABIN388789) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Background publications Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).

Crouau-Roy, Amadou, Bouissou et al.: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).

Looijenga, Stoop, de Leeuw et al.: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).

Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).

Hosts (141), (68), (12), (10)
Reactivities (203), (84), (45), (29), (29), (28), (8), (5), (3), (3), (2), (2), (1), (1)
Applications (167), (83), (46), (31), (30), (24), (20), (18), (7), (6), (6), (3), (3), (1), (1)
Conjugates (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (17), (16), (11), (7), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top