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OCT4 antibody (POU Class 5 Homeobox 1) (AA 110-141)

Details for Product anti-POU5F1 Antibody No. ABIN388789, Supplier: Log in to see
Antigen
  • POU5F1L
  • POU5F1
  • OCT3
  • OCT4
  • OTF-3
  • OTF3
  • OTF4
  • Oct-3
  • Oct-4
  • NF-A3
  • Oct-3/4
  • Oct3
  • Oct3/4
  • Oct4
  • Otf-3
  • Otf-4
  • Otf3
  • Otf3-rs7
  • Otf3g
  • Otf4
  • gp9
  • gp-9
  • oct4
  • pou2
  • POU-2
  • cb197
  • spg/pou2
  • chunp6868
  • wu:fd18d06
Epitope
AA 110-141, Glu125
36
31
30
20
13
12
8
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
297
102
45
10
10
8
5
5
5
5
3
3
3
2
1
1
Host
Rabbit
209
90
30
11
Clonality (Clone)
Polyclonal ()
Conjugate
This OCT4 antibody is un-conjugated
13
10
10
9
6
6
4
3
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
257
157
52
49
35
30
27
19
12
12
8
4
3
3
3
2
2
1
Supplier
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Immunogen This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 AA from human OCT4 (OCT3).
Clone RB14258
Isotype Ig
Specificity This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~148 amino acids surrounding E125 of human OCT3/4.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name OCT4 (OCT3) (POU5F1 Antibody Abstract)
Background Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Synonyms: POU domain class 5 transcription factor 1, POU5F1, Octamer-binding transcription factor 3, OCT-3, OTF3, OCT-4
Molecular Weight 38562 Da
Gene ID 5460
UniProt Q01860
Research Area Stem Cells, Transcription Factors, Signaling
Pathways
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (AA 110-141) (ABIN388789) Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35 µg/lane...
Immunohistochemistry (IHC) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (AA 110-141) (ABIN388789) Immunofluorescence analysis of OCT3/4 Antibody (E125) (ABIN388789) in HeLa cells. 0.0...
Background publications Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).

Looijenga, Stoop, de Leeuw et al.: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).

Crouau-Roy, Amadou, Bouissou et al.: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).

Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).