SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (C-Term), (AA 711-740) antibody

Details for Product No. ABIN388829
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Antigen
Epitope
C-Term, AA 711-740
(12), (11), (8), (8), (8), (6), (6), (2), (2), (2), (1), (1)
Reactivity
Human
(68), (26), (16), (13), (12), (12), (12)
Host
Rabbit
(52), (12), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(55), (41), (21), (15), (10), (6), (5), (5), (5), (4), (1), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN388829
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Immunogen This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711-740 AA from the C-terminal region of human SMURF1.
Clone RB4103
Isotype Ig
Specificity This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718~747 amino acids from the C-terminal region of SMURF1.
Predicted Reactivity Mouse (Murine),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SMURF1
Background SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.
Synonyms: Smad ubiquitination regulatory factor 1, Ubiquitin--protein ligase SMURF1, Smad-specific E3 ubiquitin ligase 1, hSMURF2
Molecular Weight 86113 DA
Gene ID 57154
UniProt Q9HCE7
Research Area Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (C-Term), (AA 711-740) antibody The anti-SMURF1 Pab (ABIN388829) is used in Western blot to detect SMURF1 in HL-60 cell lysate.
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (C-Term), (AA 711-740) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).

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