SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (C-Term) antibody

Antigen: SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2)

Synonyms: MGC138150, DKFZp686F0270, AI558114, AI649275, 2810411E22Rik, RGD1310067, wu:fb82a11, wu:fc41c02, LOC398372, smurf2, MGC130978, SMURF2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388831

Additional Information

Alternative name: SMURF2

Gene ID: 64750

UniProt: Q9HAU4

Immunogen: This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714~743 amino acids from the C-terminal region of human SMURF2.

Isotype: Ig

Description: Other names: E3 ubiquitin-protein ligase SMURF2, Smad ubiquitination regulatory factor 2, Smad-specific E3 ubiquitin ligase 2,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714~743 amino acids from the C-terminal region of human SMURF2.

Molecular Weight: 86113 DA

Comments:

Background: SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immnunofluorescence

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-SMURF2 Pab (ABIN388831) in 293 cell line lysate (35ug/lane). SMURF2(arrow) was detected using the purified Pab (1:30 dilution)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30.282(48):35259-35268)

Publications

Product: Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).