Ubiquitin Protein Ligase E3A (ube3a) (C-Term) antibody

Antigen: Ubiquitin Protein Ligase E3A (ube3a)

Synonyms: AS, ANCR, E6-AP, HPVE6A, EPVE6AP, FLJ26981, UBE3A, MGC92173, MGC174322, zgc:92173, im:7140733, DKFZp459N106, ube3a, Hpve6a, KIAA4216, mKIAA4216, 4732496B02, 5830462N02Rik, A130086L21Rik, As, CG6190, das, DmelCG6190, dube3a, dube3A, dUBE3A, Dube3a, MGC53073, MGC69536

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388925

Additional Information

Alternative name: UBE3A

Gene ID: 7337

UniProt: Q05086

Immunogen: This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 843~873 amino acids from the C-terminal region of human UBE3A.

Isotype: Ig

Description: Other names: Ubiquitin-protein ligase E3A, E6AP ubiquitin-protein ligase, Oncogenic protein-associated protein E6-AP, Human papillomavirus E6-associated protein

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 843~873 amino acids from the C-terminal region of human UBE3A.

Molecular Weight: 100645 DA

Comments:

Background: UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830], also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.

Synonyms: AS, ANCR, E6-AP, HPVE6A, EPVE6AP, FLJ26981, UBE3A, MGC92173, MGC174322, zgc:92173, im:7140733, DKFZp459N106, ube3a, Hpve6a, KIAA4216, mKIAA4216, 4732496B02, 5830462N02Rik, A130086L21Rik, As, CG6190, das, DmelCG6190, dube3a, dube3A, dUBE3A, Dube3a, MGC53073, MGC69536

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of UBE3A Antibody (C-term) (ABIN388925) in T47D cell line lysates (35ug/lane).UBE3A(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Malzac, Webber, Moncla et al.: "Mutation analysis of UBE3A in Angelman syndrome patients." in: American journal of human genetics, Vol. 62, Issue 6, pp. 1353-60, 1998 (PubMed).

Nuber, Schwarz, Scheffner: "The ubiquitin-protein ligase E6-associated protein (E6-AP) serves as its own substrate." in: European journal of biochemistry / FEBS, Vol. 254, Issue 3, pp. 643-9, 1998 (PubMed).

Huang, Kinnucan, Wang et al.: "Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade." in: Science (New York, N.Y.), Vol. 286, Issue 5443, pp. 1321-6, 1999 (PubMed).

Kleijnen, Shih, Zhou et al.: "The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome." in: Molecular cell, Vol. 6, Issue 2, pp. 409-19, 2000 (PubMed).

Be, Hong, Wei et al.: "Solution structure determination and mutational analysis of the papillomavirus E6 interacting peptide of E6AP." in: Biochemistry, Vol. 40, Issue 5, pp. 1293-9, 2001 (PubMed).