Sequestosome 1 (SQSTM1) (C-Term) antibody

Antigen: Sequestosome 1 (SQSTM1)

Synonyms: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, Osi, STAP, OSF-6, SQSTM1, MGC127197, MGC64432, sb:cb621, zgc:85784, DKFZp468L2213, sqstm1, MGC79491

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388979

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: SQSTM1 (p62) (C-term)

Gene ID: 8878

Swiss-Prot: Q13501

Immunogen: This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of human SQSTM1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SQSTM1.

Molecular Weight: 47687 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Cycle, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hSQSTM1-C331 (ABIN388979) in A2058 cell line lysates (35ug/lane). SQSTM1 (arrow) was detected using the purified Pab.

Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ) (ABIN388979). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.