BMI1 antibody (C-Term)

Catalog No. ABIN389084

The Rabbit Polyclonal anti-BMI1 antibody has been validated for WB and IHC (p). It is suitable to detect BMI1 in samples from Human. There are 3+ publications available.

Quick Overview for BMI1 antibody (C-Term) (ABIN389084)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB02926

Product Details anti-BMI1 Antibody

Binding Specificity: AA 257-286, C-Term

Predicted Reactivity: B, C, M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human BMI1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-BMI1 antibody (ABIN389084)

Wang, Venugopal, Manoranjan, McFarlane, OFarrell, Nolte, Gunnarsson, Hollenberg, Kwiecien, Northcott, Taylor, Hawkins, Singh: "Sonic hedgehog regulates Bmi1 in human medulloblastoma brain tumor-initiating cells." in: Oncogene, Vol. 31, Issue 2, pp. 187-99, (2012) (PubMed).

Lewis, Segditsas, Deheragoda, Pollard, Jeffery, Nye, Lockstone, Davis, Clark, Stamp, Poulsom, Wright, Tomlinson: "Severe polyposis in Apc(1322T) mice is associated with submaximal Wnt signalling and increased expression of the stem cell marker Lgr5." in: Gut, Vol. 59, Issue 12, pp. 1680-6, (2010) (PubMed).

Sangiorgi, Capecchi: "Bmi1 lineage tracing identifies a self-renewing pancreatic acinar cell subpopulation capable of maintaining pancreatic organ homeostasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 17, pp. 7101-6, (2009) (PubMed).

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.

Molecular Weight: 36949

Gene ID: 648

NCBI Accession: NP_001190991, NP_005171

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy