BMI (C-Term) antibody

Antigen: BMI

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389084

Additional Information

Gene ID: 648

UniProt: P35226

Immunogen: This BMI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the C-terminal region of human BMI.

Isotype: Ig

Description: Other names: Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BMI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the C-terminal region of human BMI.

Molecular Weight: 36949 DA

Comments:

Background: BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Stem Cells, Protein Modifications, Cancer, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hBMI1-C271 antibody(ABIN389084) in 293 cell line lysates (35ug/lane). BMI1(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

BMI1 Antibody (C271) (ABIN389084) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).

Publications

Product: Bárdos, Saurin, Tissot et al.: "HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28785-92, 2000 (PubMed).

Xia, Anderson, Diaz et al.: "MLL repression domain interacts with histone deacetylases, the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 14, pp. 8342-7, 2003 (PubMed).