Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1) (C-Term) antibody

Antigen: Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1)

Synonyms: TRF, PIN2, TRF1, TRBF1, t-TRF1, FLJ41416, hTRF1-AS, MGC109063, wu:fb74c04, TERF1, ERF1, DKFZp459A0713

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389240

Additional Information

Alternative name: TRF (TERF1)

Gene ID: 7013

UniProt: P54274

Immunogen: This TRF (TERF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344~374 amino acids from the C-terminal region of human TERF1.

Isotype: Ig

Description: Other names: Telomeric repeat binding factor (NIMA-interacting) 1, TERF1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TRF (TERF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344~374 amino acids from the C-terminal region of human TERF1.

Molecular Weight: 50246 DA

Comments:

Background: TERF1 is a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-TERF1(C-term) Pab (ABIN389240) in Jurkat cell line lysates (35ug/lane). TERF1(C-term)(arrow) was detected using the purified Pab (1:60dilution).

Publications

Product: Etheridge, Compton, Barrientos et al.: "Tethering telomeric double- and single-stranded DNA-binding proteins inhibits telomere elongation." in: The Journal of biological chemistry, Vol. 283, Issue 11, pp. 6935-41, 2008 (PubMed).

Kim, Kang, Nam et al.: "Regulation of telomeric repeat binding factor 1 binding to telomeres by casein kinase 2-mediated phosphorylation." in: The Journal of biological chemistry, Vol. 283, Issue 20, pp. 14144-52, 2008 (PubMed).