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Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (Center) antibody

Antigen

Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1)

Synonyms
HSPD1, DKFZp459H2320, HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, hld4, cpn60, groel, hsp60, hsp65, spg13, chaperonin, cb863, fa04a05, fb22d10, fi27b05, sb:cb144, id:ibd2197, wu:fa04a05, wu:fb22 ... show more
Binding Site
Alternatives

Center

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN389380
Quantity 400 µL  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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Additional Information

Alternative name HSPD1
Gene ID 3329
UniProt P10809
Immunogen This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~223 amino acids from the Center region of human HSPD1.
Isotype Ig
Description Other names: 60 kDa heat shock protein, mitochondrial, Heat shock protein 60, 60 kDa chaperonin, Chaperonin 60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HuCHA60, HSP60
Characteristics Purified Rabbit Polyclonal Antibody (Pab)
Specificity This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~223 amino acids from the Center region of human HSPD1.
Molecular Weight 61055 DA
Comments

Background: HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.

Synonyms HSPD1, DKFZp459H2320, HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, hld4, cpn60, groel, hsp60, hsp65, spg13, chaperonin, cb863, fa04a05, fb22d10, fi27b05, sb:cb144, id:ibd2197, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05, MIF4, MNA2, mopA, groL, crpA

Application Details

Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Stem Cells
Restrictions For Research Use only

Publications

Product Venner, Singh, Gupta: "Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families." in: DNA and cell biology, Vol. 9, Issue 8, pp. 545-52, 1991 (PubMed).

Hansen, Bross, Westergaard et al.: "Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter." in: Human genetics, Vol. 112, Issue 1, pp. 71-7, 2002 (PubMed).

Alternatives

Alternatives for antigen "Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1)", type "Antibodies"
Hosts Mouse (80), Rabbit (78), Goat (3)
Reactivities Human (140), Rat (Rattus) (59), Mouse (Murine) (56), Cow (Bovine) (31), Dog (Canine) (31), Horse (Equine) (18), Monkey (14), Chicken (13), Hamster (7), Rabbit (7), Guinea Pig (6), Xenopus laevis (5), Fruit Fly (Drosophila melanogaster) (4), Pig (Porcine) (4), Plasmodium falciparum (4), Chlamydia trachomatis (3), Mycobacteria (3), Sheep (Ovine) (3), All Species (2), Chimpanzee (2), Escherichia coli (E. coli) (2), General (2), Mycobacterium bovis (M. bovis) (2), Bacteria (1), Cat (Feline) (1), Dolphin (1), Helicobacter pylori (1), Insect (1), Mammalian (1), Salmonella typhimurium (S. typhimurium) (1), Snake (1), Spinach (Spinacia oleracea) (1), Trout (1), Whitefly (1), Yeast (1), Yeast (Pichia pastoris) (1)
Applications Western Blotting (WB) (137), ELISA (59), Immunofluorescence (IF) (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Immunoprecipitation (IP) (38), Immunohistochemistry (IHC) (32), Flow Cytometry (FACS) (28), Immunocytochemistry (ICC) (10), Electrophoretic Mobility-Shift Assay (EMSA) (8), Immunohistochemistry (Fixed) (IHC (fx)) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Enzyme Immunoassay (EIA) (2), Radioimmunoassay (RIA) (2), Functional Studies (Func) (1), Immunhistochemisch (Frozen Sections) (IHC (fro)) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (7), PE (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (5), AA 1-573 (4), AA 383-447 (4), AA 383-419 (2), AA 517-522 (2), Asp307 (2), N-Term (2), AA 117-122 (1), AA 150-200 (1), AA 211-288 (1), AA 31-50 (1), AA 31-547 (1), AA 350-400 (1), Asp85 (1), Internal Region (1)