|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody
|Synonyms||FP497, MGC15319, MGC42355, AI875481, AU022477, 2700049M22Rik, BID, MGC140420, bid, si:ch211-238n5.6, xbid, fp497, DKFZp469E066|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Phospho-Bid-S65 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.|
|Description||Other names: BH3-interacting domain death agonist|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Phospho-Bid-S65 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.|
|Molecular Weight||26836 DA|
Background: Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Gong, Choi, Franzin et al.: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004 (PubMed).
García-Sáez, Mingarro, Pérez-Payá et al.: "Membrane-insertion fragments of Bcl-xL, Bax, and Bid." in: Biochemistry, Vol. 43, Issue 34, pp. 10930-43, 2004 (PubMed).
Broaddus, Dansen, Abayasiriwardana et al.: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005 (PubMed).
Weng, Li, Xu et al.: "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10491-500, 2005 (PubMed).
Liu, Durrant, Yang et al.: "The interaction between tBid and cardiolipin or monolysocardiolipin." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 865-70, 2005 (PubMed).