BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody

Antigen: BH3 Interacting Domain Death Agonist (BID)

Synonyms: FP497, MGC15319, MGC42355, AI875481, AU022477, 2700049M22Rik, BID, MGC140420, bid, si:ch211-238n5.6, xbid, fp497, DKFZp469E066

Binding Site: pSer65

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389523

Additional Information

Alternative name: Bid

Gene ID: 637

UniProt: P55957

Immunogen: This Phospho-Bid-S65 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.

Isotype: Ig

Description: Other names: BH3-interacting domain death agonist

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-Bid-S65 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.

Molecular Weight: 26836 DA

Comments:

Background: Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-Phospho-Bid-S65 Pab (ABIN389523) is used in Western blot to detect Phospho-Bid-S65 in Jurkat tissue lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Gong, Choi, Franzin et al.: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004 (PubMed).

García-Sáez, Mingarro, Pérez-Payá et al.: "Membrane-insertion fragments of Bcl-xL, Bax, and Bid." in: Biochemistry, Vol. 43, Issue 34, pp. 10930-43, 2004 (PubMed).

Broaddus, Dansen, Abayasiriwardana et al.: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005 (PubMed).

Weng, Li, Xu et al.: "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10491-500, 2005 (PubMed).

Liu, Durrant, Yang et al.: "The interaction between tBid and cardiolipin or monolysocardiolipin." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 865-70, 2005 (PubMed).