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BID antibody (BH3 Interacting Domain Death Agonist) (pSer65)

Details for Product anti-BID Antibody No. ABIN389523, Supplier: Login to see
Antigen
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
Alternatives
anti-Human BID antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
pSer65
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34
33
17
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11
9
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5
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3
3
3
3
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2
2
1
1
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1
1
1
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Reactivity
Human
283
133
56
2
1
Host
Rabbit
279
70
22
10
Clonality (Clone)
Polyclonal ()
Conjugate
This BID antibody is un-conjugated
20
18
13
8
7
7
4
3
3
3
3
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3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
292
170
109
65
38
35
28
20
13
12
10
3
2
1
1
1
1
1
Supplier
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Immunogen This Bid Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.
Clone RB7105
Isotype Ig
Specificity This Phospho-Bid-S65 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name Bid (BID Antibody Abstract)
Background Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
Synonyms: BH3-interacting domain death agonist
Molecular Weight 26836 DA
Gene ID 637
UniProt P55957
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.44 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (pSer65) (ABIN389523) The anti-Phospho-Bid-S65 Pab (ABIN389523) is used in Western blot to detect Phospho-B...
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (pSer65) (ABIN389523) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Liu, Durrant, Yang et al.: "The interaction between tBid and cardiolipin or monolysocardiolipin." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 865-70, 2005 (PubMed).

Weng, Li, Xu et al.: "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10491-500, 2005 (PubMed).

Broaddus, Dansen, Abayasiriwardana et al.: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005 (PubMed).

García-Sáez, Mingarro, Pérez-Payá et al.: "Membrane-insertion fragments of Bcl-xL, Bax, and Bid." in: Biochemistry, Vol. 43, Issue 34, pp. 10930-43, 2004 (PubMed).

Gong, Choi, Franzin et al.: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004 (PubMed).