BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody

Details for Product No. ABIN389523
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Antigen
Synonyms BID, bid, si:ch211-238n5.6, fp497, xbid, DKFZp469E066, FP497, 2700049M22Rik, AI875481, AU022477
Epitope
pSer65
(35), (23), (22), (20), (15), (8), (7), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(188), (113), (54), (1), (1)
Host
Rabbit
(206), (50), (7), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(11), (11), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(209), (129), (62), (59), (40), (32), (17), (16), (15), (15), (3), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN389523
291.50 $
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Immunogen This Bid Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.
Clone RB7105
Isotype Ig
Specificity This Phospho-Bid-S65 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name Bid
Background Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
Synonyms: BH3-interacting domain death agonist
Molecular Weight 26836 DA
Gene ID 637
UniProt P55957
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.44 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody The anti-Phospho-Bid-S65 Pab (ABIN389523) is used in Western blot to detect Phospho-Bid-S65 in Jurkat tissue lysate
anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Gong, Choi, Franzin et al.: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004 (PubMed).

García-Sáez, Mingarro, Pérez-Payá et al.: "Membrane-insertion fragments of Bcl-xL, Bax, and Bid." in: Biochemistry, Vol. 43, Issue 34, pp. 10930-43, 2004 (PubMed).

Broaddus, Dansen, Abayasiriwardana et al.: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005 (PubMed).

Weng, Li, Xu et al.: "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10491-500, 2005 (PubMed).

Liu, Durrant, Yang et al.: "The interaction between tBid and cardiolipin or monolysocardiolipin." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 865-70, 2005 (PubMed).

Validation Images
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