Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (S257) antibody

Antigen: Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)

Synonyms: MCH2, Mch2, mCASP-6, MGC93335, xCaspase-6, mch2, caspase-6, CASP6, MGC127633, zgc:112960, casp6

Epitope: S257

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389525

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Phospho-Caspase 6-S257

Gene ID: 839

Swiss-Prot: P55212

Immunogen: This Phospho-Caspase 6-S257 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human caspase 6.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Caspase-6 [Precursor], CASP-6, Apoptotic protease Mch-2, CASP6, MCH2,

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human caspase 6.

Molecular Weight: 33310 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Phospho-Caspase-pS257 (ABIN389525) in liver cell line lysates (35ug/lane). Casp6-pS257(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.