Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (T506) antibody

Antigen: Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A)

Synonyms: CDC25A2, D9Ertd393e, CDC25A, cdc25a2, cdc25a, MGC115549

Epitope: T506

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389532

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Phospho-CDC25A-T506

Swiss-Prot: P30304

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T506 of human Cdc25A.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A,

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T506 of human Cdc25A.

Application Details

Application Notes: The suggested dilution is: ELISA 1:1,000 Western blotting 1:100~500 Immunohistochemistry 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

The anti-Phospho-CDC25A-T506 Pab (ABIN389532) is used in Western blot to detect Phospho-CDC25A-T506 in cells transfected with wild type or mutant T506A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Publications: Chou, Yen, Lee et al.: "The pro-apoptotic role of CDC25A: Activation of cyclin B1/CDC2 by the CDC25A C-terminal domain." in: The Journal of biological chemistry, 2010 (PubMed).

Kasahara, Goto, Enomoto et al.: "14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage." in: The EMBO journal, 2010 (PubMed).