cJun (pSer63) antibody

Antigen: cJun

Binding Site: pSer63

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389545

Additional Information

Gene ID: 3725

UniProt: P05412

Immunogen: This Phospho-cJun-S63 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.

Isotype: Ig

Description: Other names: Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-cJun-S63 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.

Molecular Weight: 35676 DA

Comments:

Background: The gene for cJun is the putative transforming gene of avian sarcoma virus 17. The cJun protein is a transcription factor highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene maps to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Dot Blot ~~ 1:500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-cJun-S63 Antibody (ABIN389545) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of anti-Phospho-cJun-pS63 Pab (ABIN389545) in mouse brain tissue lysate (35ug/lane). Mouse Phospho-cJun-pS63(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Bladh, Lidén, Dahlman-Wright et al.: "Identification of endogenous glucocorticoid repressed genes differentially regulated by a glucocorticoid receptor mutant able to separate between nuclear factor-kappaB and activator protein-1 repression." in: Molecular pharmacology, Vol. 67, Issue 3, pp. 815-26, 2005 (PubMed).

Quan, He, Voorhees et al.: "Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts." in: The Journal of biological chemistry, Vol. 280, Issue 9, pp. 8079-85, 2005 (PubMed).

Cheung, Acevedo, Cole et al.: "Altered pharmacology and distinct coactivator usage for estrogen receptor-dependent transcription through activating protein-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 3, pp. 559-64, 2005 (PubMed).

Cheng, Perkins, Yeh: "Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14492-8, 2005 (PubMed).