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cJun (pSer63) antibody
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (Dot), ELISA
|4 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Phospho-cJun-S63 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.|
|Description||Other names: Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN,|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Phospho-cJun-S63 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.|
|Molecular Weight||35676 DA|
Background: The gene for cJun is the putative transforming gene of avian sarcoma virus 17. The cJun protein is a transcription factor highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene maps to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Dot Blot ~~ 1:500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Bladh, Lidén, Dahlman-Wright et al.: "Identification of endogenous glucocorticoid repressed genes differentially regulated by a glucocorticoid receptor mutant able to separate between nuclear factor-kappaB and activator protein-1 repression." in: Molecular pharmacology, Vol. 67, Issue 3, pp. 815-26, 2005 (PubMed).
Quan, He, Voorhees et al.: "Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts." in: The Journal of biological chemistry, Vol. 280, Issue 9, pp. 8079-85, 2005 (PubMed).
Cheung, Acevedo, Cole et al.: "Altered pharmacology and distinct coactivator usage for estrogen receptor-dependent transcription through activating protein-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 3, pp. 559-64, 2005 (PubMed).
Cheng, Perkins, Yeh: "Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14492-8, 2005 (PubMed).
|Applications||ELISA (3), Western Blotting (WB) (3), Immunohistochemistry (IHC) (2), Dot Blot (Dot) (1), Immunofluorescence (IF) (1)|
|Epitopes||pSer63 (1), pSer73 (1)|