Retinoblastoma-Like 1 (p107) (RBL1) (S975) antibody

Antigen: Retinoblastoma-Like 1 (p107) (RBL1)

Synonyms: PRB1, p107, CP107, MGC40006, AW547426, prb1, MGC84607, RBL1, fj58e06, wu:fj58e06

Epitope: S975

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389640

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Phospho-Rb-like-1-S975

Gene ID: 5933

Swiss-Prot: P28749

Immunogen: This Phospho-Rb-like-1-S975 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like 1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, PRB1, P107, RBL1,

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like 1.

Molecular Weight: 120877 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-Phospho-Rb-like-1-S975 Pab (ABIN389640) is used in Western blot to detect Phospho-Rb-like-1-S975 in A2058 tissue lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.