Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN389913

Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, Map1lc3, Mpl3, zbs559, 1010001C15Rik, Atg8, LC3b, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B
Epitope
»Alternatives pThr12
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Dot Blot (DB)
Pubmed 5 references available
Catalog no. ABIN389913
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen This LC3B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T12 of human LC3B.
Clone RB12679
Isotype Ig
Specificity This Phospho-LC3B-T12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding T12 of human LC3 (APG8b). Patent pending.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LC3B
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 DA
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Autophagy, Cell Structure
Application Notes Recommend dilutions: DB : 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.41 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Alternatives for antigen "Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B)", type "Antibodies"
Hosts (83), (13), (2)
Reactivities (95), (57), (42), (27), (26), (14), (13), (3), (2), (2), (1)
Applications (65), (29), (27), (26), (20), (19), (17), (13), (9), (7), (7), (1), (1)
Conjugates (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (17), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Beacon