Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody

Details for Product No. ABIN389913
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Antigen
Synonyms Map1lc3, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, 1010001C15Rik, Atg8, LC3b, Mpl3, zbs559
Epitope
pThr12
(26), (11), (9), (8), (6), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(116), (44), (35), (24), (24), (14), (13), (4), (2), (1)
Host
Rabbit
(106), (14), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Dot Blot (DB)
(64), (55), (31), (26), (23), (20), (20), (18), (12), (5), (2), (1), (1)
Pubmed 5 references available
Quantity 400 μL
Options
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Catalog No. ABIN389913
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Immunogen This LC3B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T12 of human LC3B.
Clone RB12679
Isotype Ig
Specificity This Phospho-LC3B-T12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding T12 of human LC3 (APG8b). Patent pending.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LC3B
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 DA
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Autophagy, Cell Structure
Application Notes DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.41 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody Immunofluorescence confocal microscopy staining of LC3B. (I) Control cells treated with PBS and serum, (II) PMA-treated cells. 1. DAPI: blue, 2. LC3B: red and 3. merge.
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody (2) (Left)Formation of LC3B puncta in phagocytosing neutrophils and(Right) in cells treated with 3-MA before incubation with opsonized E. coli, as observed by immunofluorescence confocal microscopy LC3B: red.
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody (3) Dot blot analysis of Phospho-LC3 (APG8b)- T12 Antibody (ABIN389913) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL.
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

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