Cyclin-Dependent Kinase 2 (CDK2) (pThr14) antibody

Antigen: Cyclin-Dependent Kinase 2 (CDK2)

Synonyms: p33(CDK2), A630093N05Rik, MGC849, 1810047K05Rik, 2810452K22Rik, RGD1563395, CINP, 2810452K22RIK, MGC84068, cinp, MGC166364, zgc:56598, zgc:77684, wu:fa10c02, CDK2

Binding Site: pThr14

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389996

Additional Information

Alternative name: Cdk2

Gene ID: 1017

UniProt: P24941

Immunogen: This Phospho-CDK2-pT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T14 of human CDK2.

Isotype: Ig

Description: Other names: Cell division protein kinase 2, p33 protein kinase

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-CDK2-pT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T14 of human CDK2.

Molecular Weight: 33930 DA

Comments:

Background: CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot blotting~~1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-CDK2-pT14 Antibody (ABIN389996) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Gu, Rosenblatt, Morgan: "Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15." in: The EMBO journal, Vol. 11, Issue 11, pp. 3995-4005, 1992 (PubMed).

Tsai, Harlow, Meyerson: "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase." in: Nature, Vol. 353, Issue 6340, pp. 174-7, 1991 (PubMed).

Elledge, Spottswood: "A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1." in: The EMBO journal, Vol. 10, Issue 9, pp. 2653-9, 1991 (PubMed).

Ninomiya-Tsuji, Nomoto, Yasuda et al.: "Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9006-10, 1991 (PubMed).