Solute Carrier Family 29 (Nucleoside Transporters), Member 1 (SLC29A1) (pSer254) antibody

Antigen: Solute Carrier Family 29 (Nucleoside Transporters), Member 1 (SLC29A1)

Binding Site: pSer254

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389998

Additional Information

Alternative name: ENT1 (Slc29a1)

Gene ID: 2030

UniProt: Q99808

Immunogen: This Phospho-ENT1(Slc29a1)-pS254 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 239~268 amino acids surrounding S254 of human ENT1.

Isotype: Ig

Description: Other names: Equilibrative nucleoside transporter 1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Nucleoside transporter, es-type, Solute carrier family 29 member 1, ENT1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-ENT1(Slc29a1)-pS254 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 239~268 amino acids surrounding S254 of human ENT1.

Molecular Weight: 50219 DA

Comments:

Background: ENT1 is a member of the equilibrative nucleoside transporter family. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-ENT1(Slc29a1)-pS254 Antibody (ABIN389998) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).