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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 171-200), (Center) antibody

Details for Product No. ABIN390187
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Antigen
Epitope
AA 171-200, Center
(8), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(42), (22), (17)
Host
Rabbit
(38), (5), (3), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(33), (25), (13), (10), (9), (4), (3), (1), (1), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN390187
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Immunogen This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 AA from the Central region of human WISP1.
Clone RB2115-2116
Isotype Ig
Specificity This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WISP1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name WISP1
Background Wisp1 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. Wisp1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level.
Molecular Weight 40331 DA
Gene ID 8840
UniProt WISP1_HUMAN
Research Area Cell Signaling, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 171-200), (Center) antibody Western blot analysis of WISP1 Antibody (Center) (ABIN390187) in HL60 cell line lysates (35 µg/lane). WISP1 (arrow) was detected using the purified polyclonal antibody.
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 171-200), (Center) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Xie, Nakachi, Wang et al.: "Elevated levels of connective tissue growth factor, WISP-1, and CYR61 in primary breast cancers associated with more advanced features." in: Cancer research, Vol. 61, Issue 24, pp. 8917-23, 2001 (PubMed).

Su, Overholtzer, Besser et al.: "WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase." in: Genes & development, Vol. 16, Issue 1, pp. 46-57, 2002 (PubMed).

Hocevar, Mou, Rennolds et al.: "Regulation of the Wnt signaling pathway by disabled-2 (Dab2)." in: The EMBO journal, Vol. 22, Issue 12, pp. 3084-94, 2003 (PubMed).

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