DDIT4 antibody (N-Term)

Catalog No. ABIN390207

The Rabbit Polyclonal anti-DDIT4 antibody (Clone RB12592) (ABIN390207) specifically detects DDIT4 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for DDIT4 antibody (N-Term) (ABIN390207)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB12592

Product Details anti-DDIT4 Antibody

Binding Specificity: AA 20-49, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DDIT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human DDIT4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:25. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: DDIT4

Background: REDD1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS), TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD1. Thus, REDD1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

Molecular Weight: 25371

Gene ID: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process