Secreted phosphoprotein 1 (SPP1) (C-Term) antibody

Antigen: Secreted phosphoprotein 1 (SPP1)

Synonyms: OPN, BNSP, BSPI, ETA-1, MGC110940, OP, Bsp, Eta, Opn, Ric, Opnl, Apl-1, Spp-1, AA960535, AI790405, OSP, SPP1, spp1, MGC111821

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390469

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: SPP1 (C-term)

Gene ID: 6696

Swiss-Prot: P10451

Immunogen: This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280~309 amino acids from the C-terminal region of human SPP1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Osteopontin, Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Nephropontin, Uropontin, OPN, BNSP, SPP1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPP1.

Molecular Weight: 35423 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SPP1 Antibody (C-term) (ABIN390469) in NCI-H460 cell line lysates (35ug/lane). SPP1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.