SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (Center) antibody

Antigen: SET Domain Containing (Lysine Methyltransferase) 8 (SETD8)

Synonyms: PR-SET7, AA617402, AW536475, MGC117832, 2410195B05Rik, SET8, KMT5A, SET07, PR-Set7

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390472

Additional Information

Alternative name: SETD8

Gene ID: 387893

UniProt: Q9NQR1

Immunogen: This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~257 amino acids from the Center region of human SETD8.

Isotype: Ig

Description: Other names: Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, SET domain-containing protein 8, PR/SET domain-containing protein 07, PR/SET07, PR-Set7, Lysine N-methyltransferase 5A, SET8, SET07, PRSET7, KMT5A, SETD8

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~257 amino acids from the Center region of human SETD8.

Molecular Weight: 42890 DA

Comments:

Background: SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of SETD8 antibody (Center) (ABIN390472) in 293 cell line lysates (35ug/lane). SETD8 (arrow) was detected using the purified Pab.

SETD8 Antibody (Center) (ABIN390472) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SETD8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

SETD8 Antibody (Center) (ABIN390472) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Kalakonda, Fischle, Boccuni et al.: "Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1." in: Oncogene, Vol. 27, Issue 31, pp. 4293-304, 2008 (PubMed).

Houston, McManus, Adams et al.: "Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability." in: The Journal of biological chemistry, Vol. 283, Issue 28, pp. 19478-88, 2008 (PubMed).