Voltage-Dependent Anion Channel 1 (VDAC1) (Center) antibody

Antigen: Voltage-Dependent Anion Channel 1 (VDAC1)

Synonyms: PORIN, MGC111064, PORIN-31-HL, Vdac5, AL033343, fa13f11, zgc:85830, wu:fa13f11, MGC53435, vdac1, VDAC1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390564

Additional Information

Alternative name: VDAC1

Gene ID: 7416

UniProt: P21796

Immunogen: This VDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102~132 amino acids from the Center region of human VDAC1.

Isotype: Ig

Description: Other names: Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC, VDAC1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102~132 amino acids from the Center region of human VDAC1.

Molecular Weight: 30773 DA

Comments:

Background: VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules, in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of VDAC1 Antibody (Center) (ABIN390564) in HL-60, Y79 cell line lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VDAC1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

VDAC1 Antibody (Center) (ABIN390564) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Hiller, Garces, Malia et al.: "Solution structure of the integral human membrane protein VDAC-1 in detergent micelles." in: Science (New York, N.Y.), Vol. 321, Issue 5893, pp. 1206-10, 2008 (PubMed).

Shoshan-Barmatz, Zakar, Rosenthal et al.: "Key regions of VDAC1 functioning in apoptosis induction and regulation by hexokinase." in: Biochimica et biophysica acta, Vol. 1787, Issue 5, pp. 421-30, 2009 (PubMed).