VDAC1 antibody (Middle Region)

Catalog No. ABIN3043959

Cited in 1 publication. The Rabbit Polyclonal anti-VDAC1 antibody (ABIN3043959) specifically detects VDAC1 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for VDAC1 antibody (Middle Region) (ABIN3043959)

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VDAC1 Antibody

Binding Specificity: AA 154-181, Middle Region

Purpose: Anti-VDAC/Porin/VDAC1 Antibody

Sequence: QMNFETAKSR VTQSNFAVGY KTDEFQLH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-VDAC/Porin/VDAC1 Antibody (ABIN3043959). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-VDAC1 antibody (ABIN3043959)

Shen, Chen, Zhang, Du, Bai, Zhang, Jiang, Li, Wang, Zhu: "MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148532, (2016) (PubMed).

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC1

Background:

Synonyms: Voltage-dependent anion-selective channel protein 1,VDAC-1,hVDAC1,Outer mitochondrial membrane protein porin 1,Plasmalemmal porin,Porin 31HL,Porin 31HM,VDAC1,VDAC,

Tissue Specificity: Heart, liver and skeletal muscle.

Background: The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.

Sequence Similarities: Belongs to the eukaryotic mitochondrial porin family.

Molecular Weight: 31 kDa

Gene ID: 7416

UniProt: P21796