Cytokeratin 13 (KRT13) (N-Term) antibody

Antigen: Cytokeratin 13 (KRT13)

Synonyms: K13, CK13, MGC3781, MGC161462, Krt1-13, Krt-1.13

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390662

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: KRT13 (N-term)

Gene ID: 3860

Swiss-Prot: P13646

Immunogen: This KRT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115~144 amino acids from the N-terminal region of human KRT13.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Keratin, type I cytoskeletal 13, Cytokeratin-13, CK-13, Keratin-13, K13, KRT13

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human KRT13.

Molecular Weight: 49586 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of KRT13 antibody (N-term) (ABIN390662) in 293 cell line lysates (35ug/lane). KRT13 (arrow) was detected using the purified Pab.

KRT13 Antibody (N-term) (ABIN390662) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

KRT13 Antibody (N-term) (ABIN390662) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.