Phosphoglycerate Kinase 1 (PGK1) (AA 117-145), (Center) antibody

Details for Product No. ABIN391135
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Antigen
Synonyms PGK1, PGK, MIG10, PGKA, Pgk-1, Pgk, wu:fd59b07, wu:fj36g06, zgc:56252, zgc:77899, phosphoglycerate kinase 1
Epitope
AA 117-145, Center
(8), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(71), (37), (24), (14), (14), (14), (13), (3), (2), (2), (1)
Host
Rabbit
(64), (10), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(66), (39), (24), (11), (10), (6), (5), (5), (5), (3), (3), (3), (3), (2)
Pubmed 1 reference available
Quantity 400 μL
Options
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Catalog No. ABIN391135
238.50 $
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Immunogen This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 AA from the Central region of human PGK1.
Clone RB05463
Isotype Ig
Specificity This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.
Predicted Reactivity Monkey,Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PGK1
Background Synonyms: Phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2, PGKA
Molecular Weight 44483 DA
Gene ID 5230
UniProt P00558
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 117-145), (Center) antibody Western blot analysis of hPGK1-G132 (ABIN391135) in Hela, HepG2 cell line lysates (35 µg/lane). PGK1 (arrow) was detected using the purified polyclonal antibody.
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 117-145), (Center) antibody (2) Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (G132) (ABIN391135). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 117-145), (Center) antibody (3) Subcellular fractionation of HEK-293T cells transiently transfected with myc-tagged WT or mutant LRRK2 and analyzed by Western blotting. Enrichment of each fraction was assessed by stripping the membrane and reprobing with the indicated marker antibody. Two micrograms of protein per lane was loaded, and LRRK2 expression was evaluated by using anti-myc antibody. (PNAS 2005 Nov 15.102(46):16842-16847)
General Danshina, Geyer, Dai et al.: "Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice." in: Biology of reproduction, Vol. 82, Issue 1, pp. 136-45, 2009 (PubMed).

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