MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) (N-Term) antibody

Antigen: MAP/microtubule Affinity-Regulating Kinase 1 (MARK1)

Synonyms: MARK, KIAA1477, MGC126512, MGC126513, Emk3, AW491150, mKIAA1477, B930025N23Rik, MGC80341, MARK1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391219

Additional Information

Alternative name: MARK1

Gene ID: 4139

UniProt: Q2HIY1

Immunogen: This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of human MARK1.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of human MARK1.

Molecular Weight: 85000 DA

Comments:

Background: MARK is a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:200~500 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-MARK1 N-term Pab (ABIN391219) is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). 1:250 dilution of anti-MARK1 pab was used. Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.

Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 Antibody (N-term) (ABIN391219), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Drewes, Ebneth, Preuss et al.: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, 1997 (PubMed).