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Antibodies
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anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (Center) antibody
Maternal Embryonic Leucine Zipper Kinase (MELK) (Center) antibody
| Antigen |
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| Synonyms |
HPK38, KIAA0175, MPK38, AI327312, mKIAA0175, fb71b01, fe01f04, wu:fb71b01, wu:fe01f04, melk, MELK, Melk |
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Binding Site
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
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Application
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| Catalog no. |
ABIN391223 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
280.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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MELK
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Gene ID
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9833
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UniProt
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Q14680
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Immunogen
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This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208~238 amino acids from the center region of human MELK.
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Isotype
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Ig
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Description
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Other names: Maternal embryonic leucine zipper kinase, hMELK, Protein kinase PK38, hPK38, MELK, KIAA0175,
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Characteristics
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Purified Rabbit Polyclonal Antibody (Pab)
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Specificity
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This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208~238 amino acids from the center region of human MELK.
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Molecular Weight
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74642 DA
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Comments
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Background: MELK contains 1 protein kinase domain that belongs to the Ser/Thr protein kinase family. It phosphorylates ZNF622 and may contribute to its redirection to the nucleus. MELK may also be involved in the inhibition of spliceosome assembly during mitosis.
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Application Notes
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The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage
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Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
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Research Area
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Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure
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Restrictions
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For Research Use only
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Product
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Davezac, Baldin, Blot et al.: "Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation." in: Oncogene, Vol. 21, Issue 50, pp. 7630-41, 2002 (PubMed).
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Alternatives for antigen "Maternal Embryonic Leucine Zipper Kinase (MELK)", type "Antibodies"
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Hosts
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Rabbit (33), Goat (8), Mouse (3)
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Reactivities
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Human (41), Mouse (Murine) (23), Rat (Rattus) (19), Cow (Bovine) (3), Dog (Canine) (3)
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Applications
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Western Blotting (WB) (25), ELISA (19), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
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Epitopes
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C-Term (7), Internal Region (3), AA 637-651 (1), N-Term,AA 33-62 (1)
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Alternatives 
