PRKAA1 antibody (C-Term)

Catalog No. ABIN391303

This Rabbit Polyclonal antibody specifically detects PRKAA1 in WB. It exhibits reactivity toward Human and Mouse.It has been mentioned in 1 publication

Quick Overview for PRKAA1 antibody (C-Term) (ABIN391303)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB2520

Product Details anti-PRKAA1 Antibody

Binding Specificity: AA 479-510, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-510 amino acids from the C-terminal region of human AMPK alpha 1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:8000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PRKAA1 antibody (ABIN391303)

Liu, Wu, Huang: "Orientin protects myocardial cells against hypoxia-reoxygenation injury through induction of autophagy." in: European journal of pharmacology, Vol. 776, pp. 90-8, (2016) (PubMed).

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: AMPK alpha 1

Background: AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.

Molecular Weight: 64009

Gene ID: 5562

NCBI Accession: NP_006242, NP_996790

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect