Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (C-Term) antibody

Antigen: Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1)

Synonyms: PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391303

Additional Information

Alternative name: AMPK alpha 1

Gene ID: 5562

UniProt: Q13131

Immunogen: This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487~517 amino acids from the C-terminal region of human AMPK alpha 1.

Isotype: Ig

Description: Other names: 5'-AMP-activated protein kinase, catalytic alpha-1 chain, AMPK alpha-1 chain

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487~517 amino acids from the C-terminal region of human AMPK alpha 1.

Molecular Weight: 62793 DA

Comments:

Background: AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.

Synonyms: PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-AMPK alpha 1 C-term Pab (ABIN391303) in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

AMPKalpha1 Antibody (C494) (ABIN391303) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the AMPKalpha1 antibody detected the AMPKalpha1 protein (arrow).

Publications

Product: Zhang, Ye, Wu et al.: "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." in: Genome research, Vol. 10, Issue 10, pp. 1546-60, 2000 (PubMed).