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AMPK alpha2 (C-Term) antibody
Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484~514 amino acids from the C-terminal region of human AMPK.|
|Description||Other names: 5'-AMP-activated protein kinase, catalytic alpha-2 chain, AMPK alpha-2 chain|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484~514 amino acids from the C-terminal region of human AMPK.|
|Molecular Weight||62319 DA|
Background: AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of anti-AMPK alpha2 C-term Pab (ABIN391306) in mouse heart tissue lysate. AMPK alpha2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.|
Browne, Finn, Proud: "Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12220-31, 2004 (PubMed).
Andersson, Filipsson, Abbott et al.: "AMP-activated protein kinase plays a role in the control of food intake." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12005-8, 2004 (PubMed).
Shaw, Kosmatka, Bardeesy et al.: "The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 10, pp. 3329-35, 2004 (PubMed).
Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).