AMPK alpha2 (C-Term) antibody

Antigen: AMPK alpha2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391306

Additional Information

Gene ID: 5563

UniProt: P54646

Immunogen: This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484~514 amino acids from the C-terminal region of human AMPK.

Isotype: Ig

Description: Other names: 5'-AMP-activated protein kinase, catalytic alpha-2 chain, AMPK alpha-2 chain

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484~514 amino acids from the C-terminal region of human AMPK.

Molecular Weight: 62319 DA

Comments:

Background: AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-AMPK alpha2 C-term Pab (ABIN391306) in mouse heart tissue lysate. AMPK alpha2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Publications

Product: Browne, Finn, Proud: "Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12220-31, 2004 (PubMed).

Andersson, Filipsson, Abbott et al.: "AMP-activated protein kinase plays a role in the control of food intake." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12005-8, 2004 (PubMed).

Shaw, Kosmatka, Bardeesy et al.: "The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 10, pp. 3329-35, 2004 (PubMed).

Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).