PRKAA2 antibody (Internal Region)

Catalog No. ABIN571224

Product Details anti-PRKAA2 Antibody

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: PRKAA2

Sequence: CPLDALNTTK P

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CPLDALNTTKP, from the internal region of the protein sequence according to NP_006243.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65 kDa band observed in Human Heart and Liver lysates and in Mouse and Rat Heart lysates (calculated MW of 62.3 kDa according to NP_006243.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: PRKAA2 (PRKAA2 Products)

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, AMPKA2 antibody, ampk antibody, ampk2 antibody, prkaa antibody, prkaa1 antibody, PRKAA2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog antibody, PRKAA2 antibody, Prkaa2 antibody, prkaa2.S antibody, prkaa2 antibody

Background: PRKAA2, protein kinase, AMP-activated, alpha 2 catalytic subunit, AMPK, AMPK2, PRKAA, 5'-AMP-activated protein kinase, catalytic alpha-2 chain, AMP-activated protein kinase alpha 2 catalytic subunit, AMPK-alpha-2 chain

Gene ID: 5563, 108079, 78975

NCBI Accession: NP_006243

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect