Sphingosine Kinase 2 (SPHK2) (C-Term) antibody

Antigen: Sphingosine Kinase 2 (SPHK2)

Synonyms: C76851, MGC102297, CG2159, CG32484, DmelCG32484, SK2, Sphk2, DKFZp469F224

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391349

Additional Information

Alternative name: SPHK2

UniProt: Q9NRA0

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPHK2.

Isotype: Ig

Description: Other names: Sphingosine kinase 2, SK 2, SPK 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598~627 amino acids from the C-terminal region of human SPHK2.

Comments:

Background: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Application Details

Application Notes: The suggested dilution is: ELISA 1:1,000 Western blotting 1:100~500 Immunohistochemistry 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Cancer, Cell Cycle

Restrictions: For Research Use only

Images

The anti-SphK2 Pab (ABIN391349) is used in Western blot (Lane 1) to detect c-myc-tagged SphK2 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 2). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

SPHK2 Antibody (A605) (ABIN391349) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).

Publications

Product: Shutler, Glassco, Kang et al.: "Genomic structure of the human D-site binding protein (DBP) gene." in: Genomics, Vol. 34, Issue 3, pp. 334-9, 1996 (PubMed).

Liu, Sugiura, Nava et al.: "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19513-20, 2000 (PubMed).