SPHK2 antibody (C-Term)

Catalog No. ABIN391349

There are 2+ publications for this product available. The Rabbit Polyclonal anti-SPHK2 antibody is suitable to detect SPHK2 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for SPHK2 antibody (C-Term) (ABIN391349)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB03625

Product Details anti-SPHK2 Antibody

Binding Specificity: AA 590-620, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P-Leica: 1:250. IHC-P-Leica: 1:250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SPHK2 antibody (ABIN391349)

Sun, Zhang, Wang, Wang, Ma, Lei, Zhou, Sun, Lu, Liu, Han: "Down-regulation of Sphk2 suppresses bladder cancer progression." in: Tumour biology, (2015) (PubMed).

Coste, Brenneis, Linke, Pierre, Maeurer, Becker, Schmidt, Gao, Geisslinger, Scholich: "Sphingosine 1-phosphate modulates spinal nociceptive processing." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32442-51, (2008) (PubMed).

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: SPHK2

Background: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Molecular Weight: 69217

NCBI Accession: NP_001191087, NP_001191088, NP_001191089, NP_064511

UniProt: Q9NRA0

Pathways: VEGF Signaling