Vaccinia Related Kinase 1 (VRK1) (Center) antibody

Antigen: Vaccinia Related Kinase 1 (VRK1)

Synonyms: MGC117401, MGC138280, MGC142070, 51PK, vrk1, MGC64390, MGC75762, zgc:56266, wu:fe16f06, VRK1, MGC128186

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391605

Additional Information

Alternative name: VRK1

Gene ID: 7443

UniProt: Q99986

Immunogen: This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 367~396 amino acids from the center region of human VRK1.

Isotype: Ig

Description: Other names: vaccinia related kinase 1, Serine/threonine-protein kinase VRK1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 367~396 amino acids from the center region of human VRK1.

Molecular Weight: 45476 DA

Comments:

Background: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-VRK1 Pab (ABIN391605) is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).

Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381) (ABIN391605). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene.

Publications

Product: Nezu, Oku, Jones et al.: "Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase." in: Genomics, Vol. 45, Issue 2, pp. 327-31, 1998 (PubMed).