VRK1 antibody (Internal Region)

Catalog No. ABIN6266002

The Rabbit Polyclonal anti-VRK1 antibody (ABIN6266002) specifically detects VRK1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for VRK1 antibody (Internal Region) (ABIN6266002)

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VRK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VRK1 Antibody

Binding Specificity: Internal Region

Purpose: VRK1 Antibody

Specificity: VRK1 Antibody detects endogenous levels of total VRK1.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human VRK1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:200,WB 1:1000-3000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Alternative Name: VRK1

Background:

Description: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.

Gene: VRK1

Molecular Weight: 45 kDa

Gene ID: 7443

UniProt: Q99986