Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term), (AA 690-718) antibody

Details for Product No. ABIN391674
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Antigen
Synonyms CD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM, C85791, Cd31, Pecam
Epitope
C-Term, AA 690-718
(53), (16), (13), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(399), (187), (69), (43), (30), (20), (11), (5), (2), (1)
Host
Rabbit
(301), (166), (81), (4), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(59), (46), (32), (19), (10), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(306), (207), (130), (122), (106), (79), (66), (58), (44), (20), (20), (10), (5), (4), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 400 μL
Options
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Catalog No. ABIN391674
291.50 $
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Immunogen This CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-718 AA from the C-terminal region of human CD31.
Clone RB17999
Isotype Ig
Specificity This CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 697~726 amino acids from the C-terminal region of human CD31.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CD31
Background CD31 is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
Synonyms: Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', CD_antigen=CD31, Flags: Precursor , PECAM1
Molecular Weight 82536 DA
Gene ID 5175
UniProt P16284
Research Area Stem Cells, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term), (AA 690-718) antibody Western blot analysis of CD31 antibody (C-term) (ABIN391674) in HL60 cell line lysates (35 µg/lane). CD31 (arrow) was detected using the purified polyclonal antibody.
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term), (AA 690-718) antibody (2) Formalin-fixed and paraffin-embedded human lung carcinoma with CD31 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term), (AA 690-718) antibody (3) CD31 Antibody (C-term) (ABIN391674) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Newman, Berndt, Gorski et al.: "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily." in: Science (New York, N.Y.), Vol. 247, Issue 4947, pp. 1219-22, 1990 (PubMed).

Stockinger, Gadd, Eher et al.: "Molecular characterization and functional analysis of the leukocyte surface protein CD31." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3889-97, 1991 (PubMed).

Simmons, Walker, Power et al.: "Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen." in: The Journal of experimental medicine, Vol. 171, Issue 6, pp. 2147-52, 1990 (PubMed).

Validation Images
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