Mitogen-Activated Protein Kinase 7 (MAPK7) (C-Term) antibody

Antigen: Mitogen-Activated Protein Kinase 7 (MAPK7)

Synonyms: BMK1, ERK4, ERK5, PRKM7, Erk5-T, ATMPK7, F8D23.5, F8D23_5, MAP kinase 7, MPK7

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391722

Additional Information

Alternative name: ERK5

Gene ID: 5598

UniProt: Q13164

Immunogen: This ERK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782~812 amino acids from the C-terminal region of human ERK5.

Isotype: Ig

Description: Other names: Extracellular signal-regulated kinase 5, Mitogen-activated protein kinase 7

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ERK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782~812 amino acids from the C-terminal region of human ERK5.

Molecular Weight: 88636 DA

Comments:

Background: MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ERK5 C-term Pab (ABIN391722) in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) (ABIN391722), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Lee, Ulevitch, Han: "Primary structure of BMK1: a new mammalian map kinase." in: Biochemical and biophysical research communications, Vol. 213, Issue 2, pp. 715-24, 1995 (PubMed).

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995 (PubMed).