Cyclin-Dependent Kinase 1 (CDK1) (Thr14) antibody

Antigen: Cyclin-Dependent Kinase 1 (CDK1)

Synonyms: CDK1, CDC28A, MGC111195, DKFZp686L20222, Cdc2, Cdc2a, p34, cdc2, MGC92032, zgc:92032, wu:fc30e01, CDC2, MGC134454, cdc-2, xcdc2, cdc2-a, cdc28a, p34cdc2, MGC53829

Binding Site: Thr14

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391744

Additional Information

Alternative name: CDK1

Gene ID: 983

UniProt: P06493

Immunogen: This CDK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T14 of human CDK1.

Isotype: Ig

Description: Other names: V-abl Abelson murine leukemia viral oncogene homolog 1, ABL1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T14 of human CDK1.

Molecular Weight: 34095 DA

Comments:

Background: CDK1 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CDK1(arrow) using rabbit polyclonal CDK1 Antibody (T14) (ABIN391744). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDK1 Antibody (T14) (ABIN391744), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Chow, Siu, Ho et al.: "Differential contribution of inhibitory phosphorylation of CDC2 and CDK2 for unperturbed cell cycle control and DNA integrity checkpoints." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40815-28, 2003 (PubMed).

Shapira, Ben-Izhak, Bishara et al.: "Alterations in the expression of the cell cycle regulatory protein cyclin kinase subunit 1 in colorectal carcinoma." in: Cancer, Vol. 100, Issue 8, pp. 1615-21, 2004 (PubMed).

Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).