Cyclin-Dependent Kinase Inhibitor 3 (CDKN3) (C-Term) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 3 (CDKN3)

Synonyms: KAP, CDI1, CIP2, KAP1, FLJ25787, MGC70625, CDKN3, MGC133531, 2410006H10Rik

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391764

Additional Information

Alternative name: CDKN3

Gene ID: 1033

UniProt: Q16667

Immunogen: This CDKN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183~212 amino acids from the C-terminal region of human CDKN3.

Isotype: Ig

Description: Other names: Cyclin-dependent kinase inhibitor 3, CDK2-associated dual specificity phosphatase, Kinase associated phosphatase, Cyclin-dependent kinase interacting protein 2, Cyclin-dependent kinase interactor 1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDKN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183~212 amino acids from the C-terminal region of human CDKN3.

Molecular Weight: 23805 DA

Comments:

Background: CDKN3 belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. The gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CDKN3 Pab (ABIN391764) in HL-60 cell lysate. CDKN3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Poon, Hunter: "Dephosphorylation of Cdk2 Thr160 by the cyclin-dependent kinase-interacting phosphatase KAP in the absence of cyclin." in: Science (New York, N.Y.), Vol. 270, Issue 5233, pp. 90-3, 1995 (PubMed).

Hannon, Casso, Beach: "KAP: a dual specificity phosphatase that interacts with cyclin-dependent kinases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 5, pp. 1731-5, 1994 (PubMed).

Lee, Reimer, Fang et al.: "Overexpression of kinase-associated phosphatase (KAP) in breast and prostate cancer and inhibition of the transformed phenotype by antisense KAP expression." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1723-32, 2000 (PubMed).

Yeh, Lu, Chen et al.: "Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma." in: Cancer research, Vol. 60, Issue 17, pp. 4697-700, 2000 (PubMed).