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E2F Transcription Factor 1 (E2F1) (His357) antibody

Antigen

E2F Transcription Factor 1 (E2F1)
Synonyms RBP3, E2F-1, RBAP1, RBBP3, KIAA4009, mKIAA4009, E2F1, xE2F
Binding Site
Alternatives

His357
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
5 references available
Certificates ISO 9001:2008
Catalog no. ABIN391858
Quantity 0.1 mg  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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anti-E2F Transcription Factor 1 (E2F1) (His357) antibody

Additional Information
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Alternative name E2F1
Gene ID 1869
UniProt Q01094
Immunogen This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding H357 of human E2F1.
Isotype Ig
Description Other names: Transcription factor E2F1, E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, E2F1, RBBP3
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding H357 of human E2F1.
Molecular Weight 46920 DA
Comments

Background: E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Restrictions For Research Use only

Publications
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Product Saberwal, Lucas, Janssen et al.: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004 (PubMed).

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004 (PubMed).

Wang, Hou, Mohapatra et al.: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005 (PubMed).

Joshi, Ordonez-Ercan, Dasgupta et al.: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005 (PubMed).

ODonnell, Wentzel, Zeller et al.: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005 (PubMed).

Alternatives
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Alternatives for antigen "E2F Transcription Factor 1 (E2F1)", type "Antibodies"
Hosts Rabbit (132), Mouse (39), Goat (1), Sheep (1)
Reactivities Human (168), Mouse (Murine) (74), Rat (Rattus) (64), Horse (Equine) (23), Cow (Bovine) (21), Dog (Canine) (21), Guinea Pig (18), Pig (Porcine) (18), Chicken (2), Cat (Feline) (1), Chimpanzee (1), Fruit Fly (Drosophila melanogaster) (1), Monkey (1), Xenopus laevis (1)
Applications Western Blotting (WB) (96), Immunofluorescence (IF) (78), ELISA (56), Immunoprecipitation (IP) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunohistochemistry (IHC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Gel Shift (GS) (8), Immunocytochemistry (ICC) (7), Flow Cytometry (FACS) (5), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Biotin (7), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), FITC (4), PE,Cy5.5 (4), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), HRP (3), PE (3), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer337 (14), Ser337 (13), Thr433 (12), pSer364 (10), pThr433 (7), AA 162-476 (6), pSer332 (6), AA 244-264 (5), Internal Region (4), N-Term (4), AA 223-322 (3), AA 332-390 (3), AA 223-332 (2), C-Term (2), Ser332 (2), AA 310-329 (1), AA 360-369,pSer364 (1), AA 69-223 (1), Center (1), His357 (1), Middle Region (1), pHis357 (1), pSer1073 (1), pTyr892 (1)