AXL Receptor tyrosine Kinase (AXL) (N-Term) antibody

Antigen: AXL Receptor tyrosine Kinase (AXL)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391876

Additional Information

Alternative name: AXL

Gene ID: 558

UniProt: P30530

Immunogen: This AXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~44 amino acids from the N-terminal region of human AXL.

Isotype: Ig

Description: Other names: Tyrosine-protein kinase receptor UFO precursor, AXL oncogene

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~44 amino acids from the N-terminal region of human AXL.

Molecular Weight: 97374 DA

Comments:

Background: AXL, a member of the AXL/UFO subfamily of Tyr protein kinases, may function as a signal transducer between specific cell types of mesodermal origin. This Type I membrane protein has transforming potential in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia. The protein contains 2 putative fibronectin type III domains and 2 putative immunoglobulin-like C2-type domains.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of anti-AXL N-term Pab (ABIN391876) in SKBR3 cell lysate. AXL (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Flow cytometric analysis of WiDr cells using AXL Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: OBryan, Frye, Cogswell et al.: "axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5016-31, 1991 (PubMed).

Janssen, Schulz, Steenvoorden et al.: "A novel putative tyrosine kinase receptor with oncogenic potential." in: Oncogene, Vol. 6, Issue 11, pp. 2113-20, 1991 (PubMed).

Partanen, Mäkelä, Alitalo et al.: "Putative tyrosine kinases expressed in K-562 human leukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 22, pp. 8913-7, 1991 (PubMed).

Lee, Strunk, Spritz: "A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes." in: Oncogene, Vol. 8, Issue 12, pp. 3403-10, 1993 (PubMed).