EPH Receptor A7 (EPHA7) antibody

Antigen: EPH Receptor A7 (EPHA7)

Synonyms: EHK3, HEK11, Ebk, Ehk3, Mdk1, Cek11, Hek11, EPHA7, CEPHA7, fc51g03, wu:fc51g03, si:dkey-23k6.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391900

Additional Information

Alternative name: EphA7

Gene ID: 2045

UniProt: Q15375

Immunogen: This EphA7 antibody is generated from rabbits immunized with recombinant human EphA7 protein.

Isotype: Ig

Description: Other names: Ephrin type-A receptor 7, Tyrosine-protein kinase receptor EHK-3, Eph-homology kinase-3, Receptor protein-tyrosine kinase HEK11

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EphA7 antibody is generated from rabbits immunized with recombinant human EphA7 protein.

Molecular Weight: 112096 DA

Comments:

Background: EphA7 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.[1]

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EphA7 (arrow) using rabbit polyclonal EphA7 Antibody (ABIN391900).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA7 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EphA7 antibody (ABIN391900), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Fox, Holst, Chute et al.: "cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases." in: Oncogene, Vol. 10, Issue 5, pp. 897-905, 1995 (PubMed).

Holder, Klein: "Eph receptors and ephrins: effectors of morphogenesis." in: Development (Cambridge, England), Vol. 126, Issue 10, pp. 2033-44, 1999 (PubMed).