Fibroblast Growth Factor Receptor 2 (FGFR2) (N-Term) antibody

Antigen: Fibroblast Growth Factor Receptor 2 (FGFR2)

Synonyms: Fgfr2, BEK, JWS, CEK3, CFD1, ECT1, KGFR, TK14, TK25, BFR-1, CD332, K-SAM, FLJ98662, Bek, svs, Fgfr7, Fgfr-2, Fgfr-7, KGFRTr, AU043015, AW556123, MGC102519, wu:fc56c05, FGFR2, cek3, FGF-R, FGFR2IIIb, fgfr72IIIb, FGFR, bek, jws, cfd1, ect1, kgfr, tk14, tk25, bfr-1, cd332, k-sam, xfgfr2, DKFZp469H1521

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Primate

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391965

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: FGFR2 (N-term)

Gene ID: 2263

Swiss-Prot: P21802

Immunogen: This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human FGFR2.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: fibroblast growth factor receptor 2

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human FGFR2.

Molecular Weight: 92025 DA

Synonyms: Fgfr2, BEK, JWS, CEK3, CFD1, ECT1, KGFR, TK14, TK25, BFR-1, CD332, K-SAM, FLJ98662, Bek, svs, Fgfr7, Fgfr-2, Fgfr-7, KGFRTr, AU043015, AW556123, MGC102519, wu:fc56c05, FGFR2, cek3, FGF-R, FGFR2IIIb, fgfr72IIIb, FGFR, bek, jws, cfd1, ect1, kgfr, tk14, tk25, bfr-1, cd332, k-sam, xfgfr2, DKFZp469H1521

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of anti-hFGFR2-R22 Pab (ABIN391965) in 293 cell line lysates transiently transfected with the FGFR2 gene (2ug/lane). hFGFR2-R22(arrow) was detected using the purified Pab (1:30 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).